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| Registros recuperados : 23 | |
| 2. |  | RODRIGUES, F. A.; FUGANTI-PAGLIARINI, R.; MARCOLINO-GOMES, J.; NAKAYAMA, T. J.; MOLINARI, H. B. C.; LOBO, F. P.; HARMON, F. G.; NEPAMUCENO, A. L. Daytime soybean transcriptome fluctuations during water deficit stress. BMC Genomics, v. 16, n. 505, p. 2-19, 2015.| Biblioteca(s): Embrapa Agroenergia. |
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| 3. | | RODRIGUES, F. A.; FUGANTI-PAGLIARINI, R.; MARCOLINO-GOMES, J.; NAKAYAMA, T. J.; MOLINARI, H. B. C.; LOBO, F. P.; HARMON, F. G.; NEPOMUCENO, A. L. Daytime soybean transcriptome fluctuations during water deficit stress. BMC Genomics, v. 16, n. 505, 2015. 19 p.| Biblioteca(s): Embrapa Soja. |
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| 4. | | CARANHATO, A. L. H.; GIORDANI, W.; MARINHO, J. P.; REIS, R. R.; ALEKCEVETCH, J. C.; MARIN, S. R.; MARCOLINO-GOMES, J.; NEPOMUCENO, A. L.; MERTZ-HENNING, L. M. Identificação e avaliação da expressão de genes pertencentes a família CCCH zinc finger em duas cultivares de soja sob deficit hídrico. In: JORNADA ACADÊMICA DA EMBRAPA SOJA, 11., 2016, Londrina. Resumos expandidos... Londrina: Embrapa Soja, 2016. p. 104-110. (Embrapa Soja. Documentos, 373).| Biblioteca(s): Embrapa Soja. |
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| 5. | | COUTINHO, I. D.; MERTZ-HENNING, L. M.; DOPP, S. A.; NEPOMUCENO, A. L.; MORAES, L. A. C.; MARCOLINO-GOMES, J.; RICHTER, C.; SCHWALBE, H.; COLNAGO, L. A. Identification of primary and secondary metabolites and transcriptome profile of soybean tissues during different stages of hypoxia. Data in Brief, v. 21, p. 1089-1100, 2018.| Biblioteca(s): Embrapa Instrumentação. |
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| 6. | | COUTINHO, I. D.; MERTZ-HENNING, L. M.; DÖPP, S. A.; NEPOMUCENO, A.; MORAES, L. A. C.; MARCOLINO-GOMES, J.; RICHTER, C.; SCHWALBE, H.; COLNAGO, L. A. Identification of primary and secondary metabolites and transcriptome profile of soybean tissues during different stages of hypoxia. Data in Brief, v. 21, p. 1089-100, 2018.| Biblioteca(s): Embrapa Soja. |
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| 7. | | MARCOLINO-GOMES, J.; RODRIGUES, F. A.; OLIVEIRA, M. C. N. de; FARIAS, J. R. B.; NEUMAIER, N.; ABDELNOOR, R. V.; MARCELINO-GUIMARÃES, F. C.; NEPOMUCENO, A. L. Expression patterns of GmAP2/EREB-Like transcription factors involved in soybean responses to water deficit. Plos One, v. 8, n. 5, p. 1-11, May 2013. 11 p.| Biblioteca(s): Embrapa Soja. |
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| 8. | | MARCOLINO-GOMES, J.; RODRIGUES, F. A.; FUGANTI-PAGLIARINI, R.; NAKAYAMA, T. J.; MOLINARI, H. B. C.; HARMONAND, F. G.; NEPOMUCENO, A. L. Gene expression networks and cis-elements combinatorial models in soybean: circadian clock and drought responses. In: WORKSHOP ON BIOTIC AND ABIOTIC STRESS TOLERANCE IN PLANTS, 2013, Ilhéus. The challenge for the 21st century: book of abstracts. [Brasília]: Embrapa: International Advanced Biology Consortium, 2013. p. 45-46.| Biblioteca(s): Embrapa Soja. |
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| 9. | | MARCOLINO-GOMES, J.; NAKAYAMA, T. J.; MOLINARI, H. B. C.; BASSO, M. F.; HENNING, L. M. M.; FUGANTI-PAGLIARINI, R.; HARMON, F. G.; NEPOMUCENO, A. L. Functional characterization of a putative Glycine max ELF4 in transgenic Arabidopsis and its role during flowering control. Frontiers in Plant Science, v. 8, artigo 618, 2017.| Biblioteca(s): Embrapa Agroenergia. |
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| 10. | | MARCOLINO-GOMES, J.; NAKAYAMA, T. J.; MOLINARI, H. B. C.; BASSO, M. F.; MERTZ-HENNING, L. M.; FUGANTI-PAGLIARINI, R.; HARMON, F. G.; NEPOMUCENO, A. L. Functional characterization of a putative Glycine max ELF4 in transgenic arabidopsis and its role during flowering control. Frontiers in Plant Science, v. 8, artigo 618, 2017.| Biblioteca(s): Embrapa Soja. |
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| 11. | | REIS, R. R.; NAKAYAMA, T.; MARIN, S. R. R.; MARINHO, J. P.; MARCOLINO-GOMES, J.; MERTZ-HENNING, L. M.; MOLINARI, H. B. C.; NEPOMUCENO, A. L. Engenharia genética de precisão para tolerância a seca em soja. In: CONGRESSO BRASILEIRO DE SOJA, 7.; MERCOSOJA, 2015, Florianópolis. Tecnologia e mercado global: perspectivas para soja: anais. Londrina: Embrapa Soja, 2015. 3 p. 1 CD-ROM.| Biblioteca(s): Embrapa Soja. |
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| 12. | | COUTINHO, I. D.; MERTZ-HENNING, L. M.; DOPP, S. A.; NEPOMUCENO, A.; MORAES, L. A. C.; MARCOLINO-GOMES, J.; RICHTER, C.; SCHWALBE, H.; COLNAGO, L. A. Flooded soybean metabolomic analysis reveals important primary and secondary metabolites involved involved in the hypoxia stress response and tolerance. Environmental and Experimental Botany, v. 153, p. 176-187, 2018.| Biblioteca(s): Embrapa Instrumentação. |
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| 13. | | COUTINHO, I. D.; MERTZ-HENNING, L. M.; DÖPP, S. A.; NEPOMUCENO, A.; MORAES, L. A. C.; MARCOLINO-GOMES, J.; RICHTER, C.; SCHWALBE, H.; COLNAGO, L. A. Flooded soybean metabolomic analysis reveals important primary and secondary metabolites involved in the hypoxia stress response and tolerance. Environmental and Experimental Botany, v. 153, p. 176-187, 2018.| Biblioteca(s): Embrapa Soja. |
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| 14. | | COUTINHO, I. D.; MORAES, T. B.; MERTZ-HENNING, L. M.; NEPOMUCENO, A. L.; GIORDANI, W.; MARCOLINO-GOMES, J.; SANTAGNELI, S.; COLNAGO, L. A. Integrating high-resolution and solid-state magic angle spinning NMR spectroscopy and a transcriptomic analysis of soybean tissues in response to water deficiency. In: Phytochemical Analysis, v. 28, p. 529-540, 2017.| Biblioteca(s): Embrapa Instrumentação; Embrapa Soja. |
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| 15. | | MACEDO, C. R.; FUGANTI-PAGLIARINI, R.; MARCOLINO-GOMES, J.; MOLINARI, M. D. C.; BARBOSA, D. de A.; ROSA, J. da; MARIN, S. R. R.; MERTZ-HENNING, L. M.; RECH FILHO, E. L.; NEPOMUCENO, A. L. Análise de RNASeq de genes responsivos ao déficit hídrico na fase reprodutiva da soja. In: CONGRESSO BRASILEIRO DE MELHORAMENTO DE PLANTAS, 10., 2019, Águas de Lindóia. Tema: Pesquisa e Inovação para o Desenvolvimento da Sociedade: resumos dos trabalhos. [S.l.]: Sociedade Brasileira de Melhoramento de Plantas, 2019. p. 43| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia; Embrapa Soja. |
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| 16. | | REIS, R. R.; MERTZ-HENNING, L. M.; MARCOLINO-GOMES, J.; RODRIGUES, F. A.; MARIN, S. R. R.; FUGANTI-PAGLIARINI, R.; KOLTUN, A.; GONÇALVES, L. S. A.; NEPOMUCENO, A. L. Differential gene expression in response to water deficit in leaf and root tissues of soybean genotypes with contrasting tolerance profiles. Genetics and molecular biology, v. 43, e20180290, 2020. 17 p.| Biblioteca(s): Embrapa Soja. |
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| 17. | | MARCOLINO-GOMES, J.; RODRIGUES, F. A.; FUGANTI-PAGLIARINI; BENDIX, C.; NAKAYAMA, T. J.; CELAYA, B.; MOLINARI, H. B. C.; OLIVEIRA, M. C. N. de; HARMON, F. G.; NEPOMUCENO, A. L. Diurnal oscillations of soybean circadian clock and drought responsive genes. Plos One, v. 9, n. 1, e86402, Jan. 2014. 13 p.| Biblioteca(s): Embrapa Soja. |
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| 18. | | MOLINARI, M. D. C.; FUGANTI-PAGLIARINI, R.; MARCOLINO-GOMES, J.; BARBOSA, D. de A.; MARIN, S. R. R.; MERTZ-HENNING, L. M.; NEPOMUCENO, A. L.; RECH FILHO, E. L. Flower and pod genes involved in soybean sensitivity to drought. Journal of Plant Interactions, v. 16, n. 1, p. 187-200, 2021.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia; Embrapa Soja. |
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| 19. | | RODRIGUES, F. A.; MARCOLINO-GOMES, J.; CARVALHO, J. de F. C.; NASCIMENTO, L. C. do; NEUMAIER, N.; FARIAS, J. R. B.; CARAZZOLLE, M. F.; MARCELINO, F. C.; NEPOMUCENO, A. L. Subtractive libraries for prospecting differentially expressed genes in the soybean under water deficit. Genetics and Molecular Biology, Ribeirão Preto, v. 35, n. 1, suppl., p. 304-314, May 2012.| Biblioteca(s): Embrapa Soja. |
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| 20. | | MARCOLINO-GOMES, J.; RODRIGUES, F. A.; FUGANTI-PAGLIARINI, R.; NAKAYAMA, T. J.; REIS, R. R.; FARIAS, J. R. B.; HARMON, F. G.; MOLINARI, H. B. C.; MOLINARI, M. D. C.; NEPOMUCENO, A. L. Transcriptome-wide identification of reference genes for expression analysis of soybean responses to drought stress along the day. Plos One, v. 10, n. 9, e0139051, 2015. 16 p.| Biblioteca(s): Embrapa Agroenergia; Embrapa Soja. |
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| Registros recuperados : 23 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Agroenergia; Embrapa Soja. |
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Data corrente: |
02/10/2015 |
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Data da última atualização: |
13/11/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
MARCOLINO-GOMES, J.; RODRIGUES, F. A.; FUGANTI-PAGLIARINI, R.; NAKAYAMA, T. J.; REIS, R. R.; FARIAS, J. R. B.; HARMON, F. G.; MOLINARI, H. B. C.; MOLINARI, M. D. C.; NEPOMUCENO, A. L. |
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Afiliação: |
UEL; AUTOR; AUTOR; UFV; UEL; JOSE RENATO BOUCAS FARIAS, CNPSO; U C BERKELEY - BERKELEY; HUGO BRUNO CORREA MOLINARI, CNPAE; UEL; ALEXANDRE LIMA NEPOMUCENO, CNPSO. |
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Título: |
Transcriptome-wide identification of reference genes for expression analysis of soybean responses to drought stress along the day. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Plos One, v. 10, n. 9, e0139051, 2015. |
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Páginas: |
16 p. |
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DOI: |
10.1371/journal.pone.0139051 |
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Idioma: |
Inglês |
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Conteúdo: |
The soybean transcriptome displays strong variation along the day in optimal growth conditions and also in response to adverse circumstances, like drought stress. However, no study conducted to date has presented suitable reference genes, with stable expression along the day, for relative gene expression quantification in combined studies on drought stress and diurnal oscillations. Recently, water deficit responses have been associated with circadian clock oscillations at the transcription level, revealing the existence of hitherto unknown processes and increasing the demand for studies on plant responses to drought stress and its oscillation during the day. We performed data mining from a transcriptomewide background using microarrays and RNA-seq databases to select an unpublished set of candidate reference genes, specifically chosen for the normalization of gene expression in studies on soybean under both drought stress and diurnal oscillations. Experimental validation and stability analysis in soybean plants submitted to drought stress and sampled during a 24 h timecourse showed that four of these newer reference genes (FYVE, NUDIX, Golgin-84 and CYST) indeed exhibited greater expression stability than the conventionally used housekeeping genes (ELF1-β and β-actin) under these conditions. We also demonstrated the effect of using reference candidate genes with different stability values to normalize the relative expression data from a drought-inducible soybean gene (DREB5) evaluated in different periods of the day. MenosThe soybean transcriptome displays strong variation along the day in optimal growth conditions and also in response to adverse circumstances, like drought stress. However, no study conducted to date has presented suitable reference genes, with stable expression along the day, for relative gene expression quantification in combined studies on drought stress and diurnal oscillations. Recently, water deficit responses have been associated with circadian clock oscillations at the transcription level, revealing the existence of hitherto unknown processes and increasing the demand for studies on plant responses to drought stress and its oscillation during the day. We performed data mining from a transcriptomewide background using microarrays and RNA-seq databases to select an unpublished set of candidate reference genes, specifically chosen for the normalization of gene expression in studies on soybean under both drought stress and diurnal oscillations. Experimental validation and stability analysis in soybean plants submitted to drought stress and sampled during a 24 h timecourse showed that four of these newer reference genes (FYVE, NUDIX, Golgin-84 and CYST) indeed exhibited greater expression stability than the conventionally used housekeeping genes (ELF1-β and β-actin) under these conditions. We also demonstrated the effect of using reference candidate genes with different stability values to normalize the relative expression data from a drought-inducible soybean ge... Mostrar Tudo |
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Palavras-Chave: |
Drought stress; Soybean. |
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Thesagro: |
Estiagem; Resistência à seca; Seca; Soja. |
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Thesaurus NAL: |
Drought tolerance; Soybeans; Transcriptome. |
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Categoria do assunto: |
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F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/166647/1/nepomuceno-plos-one.pdf
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Marc: |
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100 1 $aMARCOLINO-GOMES, J.
245 $aTranscriptome-wide identification of reference genes for expression analysis of soybean responses to drought stress along the day.$h[electronic resource]
260 $c2015
300 $a16 p.
520 $aThe soybean transcriptome displays strong variation along the day in optimal growth conditions and also in response to adverse circumstances, like drought stress. However, no study conducted to date has presented suitable reference genes, with stable expression along the day, for relative gene expression quantification in combined studies on drought stress and diurnal oscillations. Recently, water deficit responses have been associated with circadian clock oscillations at the transcription level, revealing the existence of hitherto unknown processes and increasing the demand for studies on plant responses to drought stress and its oscillation during the day. We performed data mining from a transcriptomewide background using microarrays and RNA-seq databases to select an unpublished set of candidate reference genes, specifically chosen for the normalization of gene expression in studies on soybean under both drought stress and diurnal oscillations. Experimental validation and stability analysis in soybean plants submitted to drought stress and sampled during a 24 h timecourse showed that four of these newer reference genes (FYVE, NUDIX, Golgin-84 and CYST) indeed exhibited greater expression stability than the conventionally used housekeeping genes (ELF1-β and β-actin) under these conditions. We also demonstrated the effect of using reference candidate genes with different stability values to normalize the relative expression data from a drought-inducible soybean gene (DREB5) evaluated in different periods of the day.
650 $aDrought tolerance
650 $aSoybeans
650 $aTranscriptome
650 $aEstiagem
650 $aResistência à seca
650 $aSeca
650 $aSoja
653 $aDrought stress
653 $aSoybean
700 1 $aRODRIGUES, F. A.
700 1 $aFUGANTI-PAGLIARINI, R.
700 1 $aNAKAYAMA, T. J.
700 1 $aREIS, R. R.
700 1 $aFARIAS, J. R. B.
700 1 $aHARMON, F. G.
700 1 $aMOLINARI, H. B. C.
700 1 $aMOLINARI, M. D. C.
700 1 $aNEPOMUCENO, A. L.
773 $tPlos One$gv. 10, n. 9, e0139051, 2015.
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Embrapa Soja (CNPSO) |
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